Coding

Part:BBa_K2729008:Design

Designed by: Hajime Fujita   Group: iGEM18_Tokyo_Tech   (2018-09-30)


ZsYellow-FMDV 2A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 43
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 289
    Illegal NgoMIV site found at 367
    Illegal NgoMIV site found at 820
    Illegal AgeI site found at 632
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

YFP is optimized for Homo Sapiens in terms of reducing the number of rare codons.


Source

YFP is a variant of GFP, which is originally isolated from the jellyfish Aequorea victoria. FMDV 2A is derived from Foot-and-mouth disease virus.

References